概括
酵母在体内高效地连接了actin introns,产生了 lariat 结构. 这些切除的内子RNA含有保存的TACTAAC盒,其中一些形式阻断逆转录.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 在RNA分离过程中.
背景情况:
- 酵母中的活性蛋白基因含有对基因表达至关重要的内基因.
- 从ADC1-actin基因融合中观察到活体中有效的actin内接.
- 了解内部处理和结构对于基因调节研究至关重要.
研究的目的:
- 描述酵母菌中切除的动因内子的形式和结构.
- 为了研究保存的UACUAAC (TACTAAC盒) 序列在内部处理中的作用.
- 为了确定切除的内核RNA中的特定化学联系.
主要方法:
- 来自酵母的多A) - 和多A) + RNA 分数的分析.
- 聚烯胺凝电泳以评估RNA大小和可移动性.
- 反转录测试用于识别RNA处理中的阻断.
- 在体内32P标记和分析切除的内核RNA结构.
主要成果:
- 鉴定出了四种不同的切除的actin内部RNA形式.
- 两种切除的内部形式在凝上表现出异常的迁移.
- 一个保存的UACUAAC序列 (TACTAAC盒) 显示了一些物种的逆转录酶阻断.
- 主要切除的内子物种被确定为具有2'-5'二结合的拉里亚特RNA.
结论:
- 酵母在体内高效地处理和消耗actin内子.
- 切除的动因内子可以形成稳定的拉里亚特结构.
- TACTAAC盒参与了拼接机制,可以导致特定的RNA结构.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...


