阿贝尔森小鼠白血病病毒转换基因产品的致命作用
S F Ziegler1, C A Whitlock, S P Goff
1Molecular Biology Institute, University of California, Los Angeles 90024.
Cell
|December 1, 1981
概括
阿贝尔森病毒导致一些动物细胞系的细胞死亡,导致转化丧失. 缺少病毒蛋白的C端部分的突变体稳定地转化细胞,没有致命的影响.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 阿贝尔森病毒通常在纤维细胞细胞系中建立稳定的转化.
- 一些动物细胞系,包括一些Balb/c/3T3亚系,在阿贝尔森病毒感染后无法保持稳定的转变.
研究的目的:
- 研究在阿贝尔森病毒感染后,在特定的动物细胞系中稳定转化失败背后的机制.
- 识别和描述能够稳定转化这些敏感细胞系而不会导致细胞死亡的阿贝尔森病毒突变.
主要方法:
- 艾伯森病毒感染NIH/3T3和Balb/c/3T3细胞系.
- 感染细胞的序列过渡和表型分析.
- 综合性前病毒和病毒转化蛋白表达的分析.
- 艾贝尔森病毒突变体的分离和特征.
主要成果:
- 在敏感细胞系中,阿贝尔森病毒感染导致细胞死亡,并在几周内恢复到非转化表型.
- 逆转与集成前病毒和阿贝尔森转化蛋白表达的丧失有关.
- 突变的阿贝尔森病毒,具有缺少C终端第三位的截断转化蛋白,实现了稳定的转化,没有致命的影响.
结论:
- 野生型阿贝尔森转化蛋白的C端部分可能导致其对某些细胞系的致命作用.
- 截断的阿贝尔森转化蛋白可能改变了蛋白激酶活性调制,防止了有害影响.
- 这项研究确定了特定的病毒蛋白域,负责细胞对转化反应的差异性反应.
相关概念视频
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Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...