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相关概念视频

Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

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相关实验视频

Updated: Jul 13, 2026

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
10:05

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

大肠杆菌RNAase P具有所需的RNA组件.

R Kole, M F Baer, B C Stark

    Cell
    |April 1, 1980
    PubMed
    概括

    核糖酶P (RNAase P) 的蛋白质或RNA成分的突变可以导致大肠杆菌的热敏感性. 这项研究确定了导致突变菌株热失活的特定组件缺陷.

    科学领域:

    • 分子生物学分子生物学
    • 酶学 是一种酶学.
    • 微生物遗传学 微生物遗传学

    背景情况:

    • 核酶P (RNAase P) 对于细菌tRNA的成熟至关重要.
    • 大肠杆菌中的热敏突变可以影响RNAase P的功能.
    • 了解RNAase P的热敏感性,可以了解酶结构与功能的关系.

    研究的目的:

    • 研究大肠杆菌热敏RNAase P突变体中热失活的分子基础.
    • 为了确定蛋白质或RNA成分是否负责热敏感性.
    • 从特定突变菌株及其转基因中鉴定RNAase P酶的特征.

    主要方法:

    • 从野生型和三个热敏大肠杆菌菌株中部分净化RNAase P.
    • 净化RNAase P制剂的热无活化特征的确定.
    • 将RNAase P分离为RNA和蛋白质组成部分,并进行体外复制.
    • 分析RNA成分数量和蛋白质成分电荷.

    主要成果:

    • 来自ts241的RNAase P的蛋白质组成部分和来自ts709的RNA组成部分赋予了热敏感性.
    • 与野生类型和ts241.1.相比,ts709突变体表现出较少量的RNA成分.

    更多相关视频

    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
    10:59

    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

    Published on: May 13, 2019

    Use of Alu Element Containing Minigenes to Analyze Circular RNAs
    13:10

    Use of Alu Element Containing Minigenes to Analyze Circular RNAs

    Published on: March 10, 2020

    相关实验视频

    Last Updated: Jul 13, 2026

    Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
    10:05

    Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

    Published on: March 5, 2019

    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
    10:59

    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

    Published on: May 13, 2019

    Use of Alu Element Containing Minigenes to Analyze Circular RNAs
    13:10

    Use of Alu Element Containing Minigenes to Analyze Circular RNAs

    Published on: March 10, 2020

  • 一种逆转菌株 (A49) 在其RNAase P蛋白成分中显示出改变的电荷.

    • 结论:

    • 影响RNAase P的蛋白质或RNA成分的突变可能导致热敏感性.
    • 在体内和体外的热敏感性都由这些组件突变赋予.
    • RNAase P的热敏感性与其蛋白质或RNA子单元的缺陷有关.