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Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: July 1, 2013

针对合成的抗体作为转化蛋白pp60srcsrc的功能分析工具

T Tamura, H Bauer, C Birr

|September 1, 1983

概括

研究人员开发了针对合成的抗体,以研究罗斯肉瘤病毒.

科学领域:

在瘤学瘤学.
病毒学病毒学
免疫学免疫学免疫学

背景情况:

鲁斯肉瘤病毒 (RSV) 编码了pp60src,这是一个转化蛋白质.
了解pp60src的功能对于癌症研究至关重要.

研究的目的:

为了产生和表征对pp60src.src的抗体.
使用这些抗体,研究pp60src的表位和功能部位.

主要方法:

针对 pp60src.src.的合成寡的抗体被提升.
检测了抗体的免疫性和特异性.
瘤携带的子血清进行了交叉反应性选.
评估了与本地pp60src及其复合体结合的抗体.

主要成果:

在8种中,有4种诱导了pp60src特定抗体.
根据水友性选择的五种,被公认为本地pp60src表位.
对三种的抗体与激酶活性部位发生反应.
当与pp50和pp90复合时,针对两个的抗体不会沉pp60src.

结论:

合成可以产生针对pp60src的功能性抗体.
水友性是识别pp60src表位的有用预测指标.
特定的抗体可以探测pp60src的激酶活性和复杂形成部位.

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