相关实验视频
Updated: Aug 10, 2026

14:23
Recombineering Homologous Recombination Constructs in Drosophila
Published on: July 13, 2013
概括
杆菌通过DNA重组改变其抗原类型. 基因转换和端粒的相互重组激活了变异特异的表面抗原基因,推动了抗原谱的进化.
科学领域:
- 分子生物学分子生物学
- 寄生虫学的寄生虫学
- 遗传学 是一个遗传学.
背景情况:
- 虫 (Trypanosoma brucei) 通过改变其表面抗原来逃避宿主免疫系统.
- 变异特异性表面抗原 (VSA) 是由位于端粒的动态基因家族编码的.
- 抗原变异依赖于复杂的DNA重排机制.
研究的目的:
- 调查Trypanosoma brucei AnTat 1.1C.中VSA基因激活背后的分子机制.
- 阐明DNA重组在VSA基因切换和曲目演变中的作用.
主要方法:
- 在不同的Trypanosoma brucei克隆中分析VSA基因结构和位置 (AnTat 1.1C,AnTat 1.1B,AnTat 1.10,AnTat 1.3B).
- 研究DNA重组事件,包括基因转换和相互重组.
- 端粒序列的特征和它们在VSA基因激活中的参与.
主要成果:
- 在Trypanosoma brucei AnTat 1.1C中的VSA基因是通过基因转换修改的混合端粒序列.
- 激活AnTat 1.1C VSA基因是通过其端粒与前一个表达部位的端粒之间的相互重组而发生的.
- 端粒DNA重组,包括基因重复和损失,有助于扩展和修改VSA谱系.
结论:
- 相互重组是Trypanosoma brucei中VSA基因激活的一个关键机制.
- 端粒DNA动态,涉及基因的增加和损失,对于试体的抗原谱的进化适应至关重要.
- 了解这些机制可以了解寄生虫的免疫逃避策略和潜在的治疗点.
相关概念视频
Recombinant DNA
Overview
Viral Recombination
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
Gene Conversion
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Recombinant DNA
Overview

