相关实验视频
Updated: Aug 19, 2026

12:31
An Ex vivo Model to Study Hormone Action in the Human Breast
Published on: January 8, 2015
概括
雄激素只在高剂量时增强乳腺癌细胞生长,通过雌激素受体作用,而不是雄激素受体. 这一发现影响了对激素驱动的癌症进展的理解.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 众所周知,雄激素会影响细胞生长,但它们在人类乳腺癌细胞系 (如MCF-7) 中的特定作用是复杂的.
- 雄激素的生理度将雄激素受体 (AR) 转移到MCF-7细胞的核中,但没有显著的线粒体效应.
研究的目的:
- 研究雄激素影响MCF-7细胞生长的机制.
- 确定雄激素是否通过雄激素受体或其他途径刺激MCF-7细胞增殖.
主要方法:
- 使用人类乳腺癌细胞系MCF-7.
- 比较安德روج因在生理和药理度对细胞生长和受体转位的影响.
- 评估DNA合成和依赖雌激素的蛋白质合成.
主要成果:
- 雄激素的药理度,但不是生理度,增强了MCF-7细胞的生长.
- 高剂量的雄激素导致雄激素受体 (AR) 和雌激素受体 (ER) 的核转位.
- 药理性雄激素显著增加了DNA和依赖雌激素的蛋白质合成.
结论:
- 雄激素不会通过雄激素受体刺激MCF-7细胞生长,主要是通过雄激素受体.
- 雌激素受体在MCF-7细胞中调解药理性雄激素的线粒体效应.
- 这表明一种间接的机制,即高剂量的雄激素与雌激素受体通路相互作用,促进乳腺癌细胞的增殖.
相关概念视频
Intracellular Hormone Receptors
Lipid-soluble hormones diffuse across the plasma and nuclear membrane of target cells to bind to their specific intracellular receptors. These receptors act as transcription factors that regulate gene expression and protein synthesis in the target cell
Internal Receptors
Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Transducer Mechanism: Nuclear Receptors
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
Secondary Messengers in Hormone Action
Water-soluble hormones cannot cross the plasma membrane, so they rely on protein receptors that span the membrane to trigger intracellular signaling pathways. These pathways then activate second messengers inside the cell, including cAMP or calcium ions.
Many hormones bind to transmembrane G protein-coupled receptors that connect to regulatory G proteins. These G proteins can then activate enzymes such as adenylyl cyclase or phospholipase C. Adenylyl cyclase converts ATP to cAMP, activating...
Many hormones bind to transmembrane G protein-coupled receptors that connect to regulatory G proteins. These G proteins can then activate enzymes such as adenylyl cyclase or phospholipase C. Adenylyl cyclase converts ATP to cAMP, activating...
Target Cell Response to Hormones
Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...

