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Updated: May 10, 2026

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

在无细胞反应中的特定位点多化

C L Moore, P A Sharp

    Cell
    |March 1, 1984
    PubMed
    概括

    这项研究表明,HeLa细胞提取物可以准确地为RNA添加多氨酸 (多A) 尾巴,这对于基因表达至关重要. 这种多基化是特定的,可能涉及小核核核糖核蛋白颗粒 (snRNP).

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    相关概念视频

    Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

    Pre-mRNA Processing: Modification of pre-mRNA Ends

    In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
    Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...