Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Chromosome Structure02:40

Chromosome Structure

22.1K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
22.1K
Homologous Recombination02:31

Homologous Recombination

58.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.9K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.1K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.1K
Gene Conversion02:08

Gene Conversion

9.2K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.2K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.7K
Crossing Over01:30

Crossing Over

6.4K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Excessive KLF2 impairs mesenchymal-epithelial transition efficiency and adhesion in endometrial stromal cells via AKT phosphorylation.

Reproductive biomedicine online·2025
Same author

Is polyethylene glycol loxenatide 100 μg the preferred glucagon-like peptide-1 receptor agonist for type 2 diabetes mellitus? A meta-analysis and trial sequential analysis.

European review for medical and pharmacological sciences·2024
Same author

[Play the "combo fist" in the diagnosis and treatment of advanced gastric cancer].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2024
Same author

Lactiplantibacillus plantarum and faecal microbiota transplantation can improve colitis in mice by affecting gut microbiota and metabolomics.

Beneficial microbes·2024
Same author

Bibliometric analysis of intestinal microbiota in diabetic nephropathy.

European review for medical and pharmacological sciences·2023
Same author

Efficacy and safety of iguratimod combined with methylprednisolone for primary Sjögren's syndrome: a meta-analysis and trial sequential analysis.

European review for medical and pharmacological sciences·2023

相关实验视频

Updated: May 5, 2026

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

11.4K

在lambda特定位点重组中的定向性决定因素.

W Bushman, S Yin, L L Thio

    Cell
    |December 1, 1984
    PubMed
    概括
    此摘要是机器生成的。

    DNA结构特征决定了lambda位点特定重组的方向性. 这种Xis蛋白是Xis蛋白.

    更多相关视频

    Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
    11:12

    Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

    Published on: September 11, 2017

    6.8K
    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
    07:55

    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

    Published on: September 11, 2022

    2.1K

    相关实验视频

    Last Updated: May 5, 2026

    Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
    11:40

    Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

    Published on: June 25, 2013

    11.4K
    Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
    11:12

    Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

    Published on: September 11, 2017

    6.8K
    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
    07:55

    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

    Published on: September 11, 2022

    2.1K

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 生物化学 生物化学

    背景情况:

    • 兰巴达特定位点重组是病毒基因组集成和切除的关键过程.
    • 这种重组的方向性受到菌体附着部位内的特定DNA序列的影响.
    • 已观察到Xis蛋白在调节整合和切除中的作用,但在分子水平上尚未完全阐明.

    研究的目的:

    • 识别和描述负责lambda位点特定重组的定向性的DNA结构特征.
    • 阐明Xis蛋白对集成和切除反应产生不同影响的机制.
    • 研究Xis蛋白的结合特性及其对Int蛋白结合的影响.

    主要方法:

    • 基因足迹测试用于识别调节蛋白的结合位.
    • 部位定向突变发生,以改变附着部位内的特定DNA序列.
    • 凝电泳和定量结合试验用于测量蛋白质-DNA相互作用.
    • 对再组合产品的分析,以确定序列改变和蛋白质结合的影响.

    主要成果:

    • 控制重组方向的关键DNA结构特征位于交叉区域的左侧超过70bp,右侧超过40bp.
    • 这些序列的空间排列 (在相同或不同的附着部位) 决定了Xis蛋白在集成 (抑制) 和切除 (刺激) 中的对立作用.
    • 希斯蛋白与左菌体臂的邻近直接重复物合作结合,诱导DNA构造变化并增强Int蛋白结合32倍.

    结论:

    • 这项研究确定了特定的DNA区域及其空间配置作为兰巴达重组的方向性的关键决定因素.
    • 希斯蛋白对整合和切除的差异性影响是由其与不同的序列安排的相互作用解释的.
    • 通过合作性DNA结合和形状变化,Xis蛋白作为Int结合的强大的调节器,从而控制重组方向性.