交配信号控制着突变的表达,这种突变是由于将可转移的重复元素插入到与各种酵母基因相邻的突变产生的
Cell
|November 1, 1980
概括
酵母菌中的ROAM突变导致基因过度产生,特别是在平分体菌株中. 这种过度生产与结合能力有关,并且涉及到基因附近的Ty元素插入.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 在Saccharomyces cerevisiae中的ROAM突变导致了基因产品的过度生产.
- 过度生产的程度在不同的酵母交配类型和性水平之间有所不同.
研究的目的:
- 研究酵母菌中ROAM突变背后的遗传基础和调控机制.
- 为了确定Ty元素在ROAM介导的基因表达中的作用.
主要方法:
- 在Saccharomyces cerevisiae中对ROAM突变的遗传分析.
- 克隆和描述ROAM突变基因CYC7-H2.2. 的特征.
- 研究影响结合的突变 (ste突变) 对ROAM表达的影响.
主要成果:
- 与非结合双胞胎相比,能够结合的单胞胎菌株的过度产量在单胞胎菌株中更高.
- 能够交配的双胞胎菌株表现出过度生产,不管它们的胞能力如何.
- 在CYC7的5'非编码区域中插入Ty1元素在ROAM突变中被发现.
- 阻止结合的ste7突变减少了ROAM过度生产.
结论:
- 罗姆突变与Ty元素的插入有关,可能是改变基因表达的原因.
- 罗姆突变似乎对调节酵母结合的信号做出反应.
- Ty元素序列可能充当结合相关调节信号的受体.
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