概括
感染瘤病毒的小鼠纤维细胞显示出明显的多变化. 病毒感染和转变改变了细胞蛋白质配置,影响了特定的分子量和糖蛋白.
科学领域:
- 细胞生物学 细胞生物学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 小鼠纤维细胞 (NIH/3T3,BALB/3T3,3T3 FL) 是研究细胞变化的模型系统.
- 瘤病毒,包括RNA瘤和白血病病毒,可以改变宿主细胞的多组成.
- 内源性瘤病毒及其糖蛋白可以存在未感染的细胞中.
研究的目的:
- 为了分析小鼠纤维细胞的多化合物组成.
- 为了研究RNA肉瘤和白血病病毒感染对细胞多的影响.
- 为了识别与病毒转化相关的蛋白质配置的变化.
主要方法:
- 采用二维凝电泳来分离和可视化细胞多.
- 试验性片映射被用来进一步描述和比较蛋白质碎片.
- 乳氧化酶催化化被用来标记细胞表面蛋白质.
主要成果:
- NIH/3T3和BALB/3T3细胞表现出类似的多图,BALB/3T3缺少两个主要的多.
- 受感染的NIH / 3T3和3T3FL细胞显示了大约73,000和80,000达尔顿的新型多.
- 莫洛尼肉瘤病毒对3T3 FL细胞的转化导致了显著的多变化,包括60,000达尔顿组件和48,000达尔顿糖蛋白的变化.
结论:
- 病毒感染会导致小鼠纤维细胞的多形状发生特定的变化.
- 莫洛尼肉瘤病毒的转变导致细胞蛋白质的广泛改变.
- 在转变细胞中观察到的重大变化与通过化标记的细胞表面蛋白质无关.
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