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在酵母酵母中的交配类型位点的体外突变分析

K Tatchell, K A Nasmyth, B D Hall

|November 1, 1981

概括

研究人员在酵母的交配类型位点 (MAT) 中产生突变,以了解细胞类型调节. 这项研究绘制了MAT位点内的关键基因图,揭示了它们的DNA序列和功能.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
酵母生物学的酵母生物学

背景情况:

在Saccharomyces cerevisiae中的交配类型位点 (MAT) 控制细胞类型的确定.
每个MAT等位基因产生两个不同的转录,调节细胞身份.

研究的目的:

在MATa和MATα位点内构建和描述体外突变.
将这些突变遗传地图和序列,以精确地定位DNA上的功能基因.

主要方法:

在体外突变利用具有限制位点 (链接器) 的合成寡核酸产生突变.
基于等离子体的突变插入到克隆的MAT DNA中.
酵母转化来补充现有的突变和基因位置的地图.

主要成果:

MATa1基因定位在Ya区域,负责a1转录.
MAT alpha 1基因映射到Y alpha和相邻的Z区域,与alpha 1转录相关.
在MATα位点的共同X区域内发现的MATα2基因.

结论:

精确地绘制MAT基因,可以了解细胞类型调节机制.
大多数突变会破坏基因翻译,但有些可能会影响转录启动.
这项工作完善了我们对酵母交配类型的复杂遗传组织的理解.

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