概括
葡萄糖皮质类药物调节小鼠乳腺瘤病毒 (MTV) 基因转录. 这项研究表明,在受感染的老鼠肝瘤细胞中,激素反应元素位于病毒DNA内,而不是侧面细胞序列.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 遗传学 是一个遗传学.
背景情况:
- 已知葡萄皮质类药物可以调节大鼠肝瘤组织培养 (HTC) 细胞中集成的小鼠乳腺瘤病毒 (MTV) 基因的转录.
- 调控这种荷尔蒙控制的调控元素的确切位置,无论是在侧面的细胞DNA中还是病毒基因组内,仍然不清楚.
研究的目的:
- 为了调查MTV基因转录的荷尔蒙调节是否来自侧边细胞序列或来自病毒DNA内部.
- 确定宿主整合部位在葡萄糖皮质激素介导的MTV基因表达中的作用.
主要方法:
- 对MTV和相邻的HTC序列在两个MTV感染的HTC细胞系 (J2.15和J2.17) 中的相对转录率的分析,其中有单个MTVDNA插入.
- 在未感染的HTC细胞中测量转录速率,使用缺乏MTV DNA的同等序列.
- 进行RNA脉冲标记实验以量化MTV基因转录率.
- 转录映射以确定核MTV RNA的5'终端.
主要成果:
- 葡萄皮质类药物刺激MTV基因转录在J2.17细胞中,但不是在J2.15细胞中.
- 在受感染或未受感染的细胞中,没有检测到来自侧边细胞序列的转录.
- 在J2.17细胞中,MTV RNA的合成不是由于来自细胞促进体的读透转录.
- 转录映射显示,MTV RNA的5'终端位于前病毒DNA内.
结论:
- 集成MTV的宿主部位不需要在转录方面活跃或对病毒基因表达有荷尔蒙反应.
- 葡萄糖皮质醇诱导的MTVRNA合成由位于前病毒DNA中的激素反应元素介导,而不是侧边细胞序列.
- 这些发现表明,集成的小鼠乳腺瘤病毒含有它自己的调节元素,对葡萄糖皮质激素反应.
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