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Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5
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Published on: January 28, 2016

简单疹病毒安普利康:一种新的真核缺陷病毒克隆增强载体

R R Spaete, N Frenkel

    Cell
    |August 1, 1982
    PubMed
    概括

    研究人员开发了一种简单疹病毒 (HSV) 片系统,用于克隆和放大真核细胞中的DNA. 这种新型的载体系统有效地复制并可以在病毒库存中传播,从而使基因转移应用成为可能.

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    Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...