相关实验视频

Updated: Jul 12, 2026

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
10:40

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

在dictyostelium discoideum中细胞类型特定的actin mRNA群体

A S Tsang, H Mahbubani, J G Williams

    Cell
    |December 1, 1982
    PubMed
    概括

    在发育过程中,Dictyostelium discoideum 的actin mRNA序列发生变化. 当细胞分化成子时,特定的actin mRNA变异会丢失,这表明基因表达差异.

    更多相关视频

    High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry06:47

    High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry

    Published on: September 10, 2018

    Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria06:08

    Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

    Published on: January 25, 2019

    相关概念视频

    Introduction to Actin01:26

    Introduction to Actin

    Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution.  Actin coding genes are conserved within species and across different species.
    Actin Polymerization01:42

    Actin Polymerization

    Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
    The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight actin...
    Actin Filament Depolymerization01:19

    Actin Filament Depolymerization

    Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
    In F-actin, the ADF/cofilin proteins...
    The Role of Actin and Myosin in Non-muscle Cells01:10

    The Role of Actin and Myosin in Non-muscle Cells

    Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They  are held...
    Actin Polymerization and Cell Motility01:13

    Actin Polymerization and Cell Motility

    Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
    Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.
    Actin Treadmilling01:18

    Actin Treadmilling

    Actin filaments undergo polymerization and depolymerization from either end. The polymerization and depolymerization rates depend on the cytosolic concentration of free G-actins. The polymerization rate is generally higher at the plus or barbed end, while the depolymerization rate is higher at the minus or pointed end. At a steady state, critical concentration describes the concentration of free G-actin monomers at which the polymerization rate at the plus end is equal to that of the...