相关实验视频

Updated: Jan 8, 2026

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
06:40

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Published on: March 19, 2018

65.9K

来自Drosophila RNA聚合酶II位点的序列的分子克隆,通过P元素转位子标记进行P元素标记

L L Searles, R S Jokerst, P M Bingham

    Cell
    |December 1, 1982
    PubMed
    概括

    研究人员在Drosophila melanogasterRNA聚合酶II中发现了由P元素插入引起的致命突变. 这一发现为克隆Drosophila中的特定基因提供了一种新方法.

    更多相关视频

    A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells11:56

    A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

    Published on: April 12, 2014

    13.9K
    Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System14:55

    Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

    Published on: May 12, 2013

    61.6K

    相关概念视频

    An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice06:40

    An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

    This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal...
    65.9K
    Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure08:40

    Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure

    A reference measurement procedure for the absolute quantification of Aβ1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is...
    8.0K
    Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function11:47

    Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

    We present a biochemical and behavioral protocol to evaluate the efficacy of mitochondria-targeted water-soluble compounds for the treatment of Spinocerebellar ataxia type 1 (SCA1) and other cerebellar neurodegenerative diseases.
    11.1K
    Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain05:51

    Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain

    Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization.
    20.3K
    A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells11:56

    A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

    Ascorbate plays numerous important roles in cellular metabolism, many of which have only come to light in recent years. Here we describe a medium-throughput, specific and inexpensive microplate assay for the determination of both intra- and extracellular ascorbate in cell culture.
    13.9K
    Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System14:55

    Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

    We describe a method to target drugs to the central nervous system by either implanting a catheter or performing a bolus injection into the right lateral ventricle in mice. We focus specifically on the delivery of antisense oligonucleotides. This technique is readily adaptable to other drugs and to...
    61.6K