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在研究通过染色体消化与微型菌核酶进行核细胞分相的研究中,还有另一个潜在的工件

J D McGhee, G Felsenfeld

|April 1, 1983

概括

核细胞核颗粒的限制酶消化意外地产生了不同的DNA片段. 这些碎片是来自内部DNA裂变的结果,而不是大量核细胞分相,揭示了依赖序列的DNA包装.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物化学生物化学

背景情况:

核细胞是真核生物中DNA包装的基本单元.
了解核细胞结构和DNA组织对于基因调节至关重要.
之前的研究表明,大量核细胞分相会影响DNA的可访问性.

研究的目的:

在限制酶裂变时研究红细胞核细胞核细胞核粒子DNA的碎片化模式.
确定观察到的DNA片段的起源及其与核细胞分相的关系.
为了阐明核子体内特定序列的DNA相互作用.

主要方法:

隔离的核细胞核颗粒的限制酶消化.
使用凝电泳分析分析DNA片段大小.
确定DNA基组成 (瓜含量) 和周期性.
对微型菌核酶裂变部位的研究.

主要成果:

限制酶裂变产生了不同的亚核素体DNA片段,与预期相反.
这些碎片起源于微型菌内部核酶裂变,而不是大量核细胞分相.
裂变发生在大约10个基对间隔,首选在富含AT的序列.
完整的145个基对DNA碎片表现出显著的关氨酸含量波动 (高达10%),周期性为10个基对.

结论:

核细胞核粒子DNA碎片化受到序列特定的裂变模式的影响.
观察到的DNA片段种群是由内部裂变机制所偏向的.
序列依赖的DNA特性,而不是批量分相,可能解释了之前的核细胞分相要求.

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