从腺病毒晚期促进体表达的SV40 T抗原的翻译控制
相关实验视频
Updated: Aug 21, 2026

15:33
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
Published on: August 13, 2013
概括
研究人员创建了腺病毒-SV40杂交病毒. 尽管RNA产量相同,但只有一个混合病毒有效地产生了SV40大T抗原,这表明受感染细胞的转录后调节.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 遗传学 是一个遗传学.
背景情况:
- 腺病毒和猿类病毒40 (SV40) 是不同的病毒,具有独特的复制策略.
- SV40 A基因编码大T抗原,这是病毒DNA复制和瘤发生的关键蛋白质.
- 腺病毒基因表达受到严格管制,特别是在晚期.
研究的目的:
- 构建和描述新型腺病毒-SV40杂交病毒.
- 为了研究SV40A基因的表达,当被置于腺病毒主要晚期促进者的控制下时.
- 确定影响腺病毒感染细胞中SV40大T抗原合成的潜在调节机制.
主要方法:
- 使用体外和体外重组构建四种腺病毒-SV40杂交病毒.
- 精确插入SV40A基因到腺病毒基因组中的特定位置.
- 在感染细胞和体外翻译系统中分析病毒RNA和蛋白质合成.
主要成果:
- 这四种混合病毒都产生了高水平的SV40编码RNA,该RNA始于腺病毒晚期促进体.
- 只有一个混合病毒 (Ad-SVR284) 在感染细胞中有效合成SV40大T抗原.
- 与Ad-SVR284.4相比,其他重组剂的产生大约是大T抗原的20倍.
- 试管体内翻译实验显示,无论病毒如何,从相同数量的混合T mRNA中产生相同的T抗原合成.
结论:
- 对SV40 A基因的高效转录并不能保证腺病毒感染细胞中高水平的大型T抗原的产生.
- 一个后转录的调节机制,可能是翻译的,在腺病毒感染的细胞中是有效的.
- 这种调节机制有选择性地影响混合病毒中SV40大T抗原的表达,并且在无细胞系统中不存在.
更多相关视频
10:13An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
Published on: January 12, 2018
06:41Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors
Published on: October 20, 2023
相关概念视频
Initiation of Translation
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
Leaky Scanning
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...