相关实验视频

Updated: Jun 10, 2026

Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry
09:45

Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry

Published on: October 19, 2016

至少有两个转移序列在不同试基因克隆中表达表面抗原基因的表达部位中相关

E Pays, S Van Assel, M Laurent

    Cell
    |September 1, 1983
    PubMed
    概括

    三生体表面抗原的基因表达包括基因重复和转移到表达部位. 一个"伴侣"序列,一个转移的DNA片段,在一些克隆中与表达链接副本一起发现,表明灵活的基因调节机制.

    更多相关视频

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp10:44

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

    Published on: June 20, 2018

    Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells09:13

    Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells

    Published on: January 17, 2019

    相关概念视频

    Overview of Transposition and Recombination02:13

    Overview of Transposition and Recombination

    Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
    DNA-only Transposons02:57

    DNA-only Transposons

    DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
    The donor site from where the transposon is excised is either degraded or...
    LTR Retrotransposons03:08

    LTR Retrotransposons

    LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
    The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
    Exon Recombination02:32

    Exon Recombination

    The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
    Exon shuffling follows “splice frame rules.” Each exon has three reading...
    Reporter Genes02:11

    Reporter Genes

    Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
    Commonly used reporter...
    Transposons01:24

    Transposons

    Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...