相关实验视频
Updated: Jul 24, 2026

11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
概括
研究人员分离了大鼠纤维内素cDNA克隆,揭示了细胞和肝素结合域中的同源序列. 替代拼接产生至少三个纤维内素信使RNA (mRNA) 从鼠肝中的单个基因.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 纤维菌素是一种关键的细胞外基质蛋白,参与细胞粘附,迁移和伤口愈合.
- 了解纤维菌素结构和基因表达对于理解其多样化的生物作用至关重要.
研究的目的:
- 为了分离和描述编码大鼠纤维蛋白的互补DNA (cDNA) 克隆.
- 为了确定大鼠纤维素C端域的序列,包括结合区域.
- 为了研究大鼠肝脏中不同纤维内素信使RNA (mRNA) 变异的分子基础.
主要方法:
- 通过使用来自老鼠肝脏库的lambda gt11表达向量来分离cDNA克隆.
- 限制映射和DNA测序以确定纤维素菌素cDNA的核酸序列.
- 绘制S1核酶映射以分析纤维内素 mRNA 种群.
主要成果:
- 成功地分离和测序了代表C-终端35%的老鼠纤维素的cDNA克隆.
- 在细胞和肝素结合域内确定了同源的重复序列.
- 证明了大鼠肝脏中至少有三种不同的纤维内素mRNA转录的存在,由于替代拼接,它们的编码潜力有所不同.
结论:
- 小鼠纤维素 mRNA 异质性源于编码区域内的替代拼接.
- 这些发现表明,单个基因编码了具有潜在不同的功能的多个纤维素蛋白异型.
- 这项研究提供了关于纤维菌素多样性的结构基础和调节机制的见解.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Fibronectins Connect Cells with ECM
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...

