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相关概念视频

Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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相关实验视频

Updated: Jul 6, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

oriX:大肠杆菌中的一个新的复制起源.

B de Massy, J Patte, J M Louarn

    Cell
    |January 1, 1984
    PubMed
    概括

    大肠杆菌的复制从多个来源开始,包括等离子体 (oriV) 和新染色体起源 (oriX),特别是在压力条件下. 这表明oriX在协调DNA复制与细胞分裂方面发挥了作用.

    科学领域:

    • 微生物学 微生物学
    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.

    背景情况:

    • 启动DNA复制对于细菌细胞的活力至关重要.
    • 在大肠杆菌中,复制的主要来源是ORIC.
    • 整合性抑制和特定突变可以改变复制模式.

    研究的目的:

    • 在特定突变条件下调查大肠杆菌染色体复制的起源.
    • 为了识别和表征除了ORIC之外的替代复制起源.
    • 探索这些起源在复制和细胞分裂中的作用.

    主要方法:

    • 在DNA突变Hfr菌株中的标记频率分析.
    • 在oxa1突变体中复制起源的表征.
    • 带有和没有集成等离子体的菌株的表型分析.

    主要成果:

    • 在42°C时,复制从集成等离子体起源 (oriV) 和新染色体起源 (oriX) 开始.
    • oriX 位于靠近预导体 rac (oriJ) 的位置,但是不同的.
    • 在一个oxa1突变体中,复制从oriV,oriX和oriC开始.
    • 失去集成等离子体会产生一种对温度敏感的菌株,其复制依赖于oriC和oriX.

    更多相关视频

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
    11:12

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

    Published on: September 11, 2017

    Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
    09:44

    Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

    Published on: December 23, 2022

    相关实验视频

    Last Updated: Jul 6, 2026

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
    11:19

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

    Published on: August 21, 2016

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
    11:12

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

    Published on: September 11, 2017

    Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
    09:44

    Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

    Published on: December 23, 2022

  • 从oriX进行复制是缓慢的,双向的.

    • 结论:

    • 大肠杆菌具有多个功能复制起源,包括新发现的oriX.
    • 在特定的压力条件下,在没有集成等离子体的情况下,oriX被激活.
    • 新型起源oriX可能参与DNA复制与细胞分裂的合.