相关实验视频
Updated: Jul 17, 2026
14:49
Associated Chromosome Trap for Identifying Long-range DNA Interactions
Published on: April 23, 2011
概括
鼠标c-abl基因被分离并进行了表征. 病毒abl (v-abl) 很可能是通过外子拼接从c-abl产生的,并且已确定了不同的功能区域.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 病毒学 病毒学
背景情况:
- 阿贝尔森小鼠白血病病毒 (A-MuLV) 含有从小鼠细胞abl (c-abl) 基因中获得的转化基因 (v-abl).
- 了解c-abl的基因组结构对于破译病毒瘤基因起源至关重要.
研究的目的:
- 为了隔离和表征小鼠c-abl基因.
- 为了研究c-abl和v-abl序列之间的关系.
- 在c-abl基因中识别功能域.
主要方法:
- 基因隔离和表征技术.
- 同源区域的DNA测序. 同源区域的DNA测序.
- 对c-abl和v-abl进行比较序列分析.
主要成果:
- 鼠标的c-abl位置大约跨越40kb,v-abl同质分布在多个群集中.
- 似乎v-abl是由c-abl产生的,主要是通过多个前体的拼接.
- 该c-abl基因含有不同的区域:氨酸激酶域和C-终端部分,不必转化能力.
结论:
- 这项研究阐明了小鼠c-abl基因的基因组组织.
- 它提供了对V-abl瘤基因的逆转录病毒捕获和演变的见解.
- 不同的外显子使用和域结构是c-abl与病毒对应物相关的关键特征.
相关概念视频
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...