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Updated: Dec 10, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

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正常和突变的人类β-环球蛋白前mRNAs在体外被忠实而有效地拼接

A R Krainer, T Maniatis, B Ruskin

    Cell
    |April 1, 1984
    PubMed
    概括

    这项研究表明,在HeLa细胞核提取物中,人体β-环球蛋白mRNA前体的体外剪接是高效和准确的. 覆盖增强了拼接特异性,导致β-thalassemia的突变在体外和体内同样影响了拼接.

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