概括
研究人员开发了一种方法,使用DNA转染来转移细胞表面分子. 这种技术成功地在受体细胞中表达了21种研究的分子中的19种,证明了广泛的适用性.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 之前的研究表明,小鼠L细胞与表达分化抗原的人类T细胞系DNA的稳定转移.
- 这些抗原的身份使用免疫沉和SDS-多烯胺凝电泳证实了.
研究的目的:
- 报告一种精细的协同传染过程,用于产生表达各种细胞表面分子的稳定传染剂.
- 评估转染方法在不同细胞类型和抗原表达的效率和适用性.
主要方法:
- 鼠标L细胞与肉丁胺激酶基因 (tk) 和整个细胞DNA的共同感染.
- 使用克桑丁-阿米诺丁-提米丁 (HAT) 介质的变换剂的选择.
- 通过色结合单克隆抗体和光激活细胞分类 (FACS) 识别和选择表达细胞.
主要成果:
- 该程序产生了表达各种细胞表面分子的变质剂,在21个研究的分子中,有19个分子成功转移.
- 表达频率大约为每10^3个胺基酶阳性 (Tk+) 变换剂中的一个.
- 重要的是,在12个分子中,有9个分子使用最初没有表达抗原的细胞的DNA表达得同样好.
结论:
- 描述的共传染和FACS选择方法有效地产生表达多种细胞表面分子的稳定变质剂.
- 该方法的有效性证明了分子的成功转移和表达,无论它们在捐赠细胞中的原始表达状态如何.
- 这种技术为研究细胞表面分子功能和分布提供了一种多功能工具.
相关概念视频
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Diversity of Antigen Receptors
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...


