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在酵母端粒中组织DNA序列和复制起源

C S Chan, B K Tye

|June 1, 1983

概括

在Saccharomyces cerevisiae中的端粒具有保存的DNA序列,具有许多复制起源. 这些序列,包括Y和X元素,显示了相关酵母物种的变化.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
酵母研究酵母研究

背景情况:

端粒可以保护染色体的末端.
了解端粒结构对于基因组稳定至关重要.

研究的目的:

描述Saccharomyces cerevisiae中与端粒相邻的DNA序列.
在这些端粒区域内识别重复元素和复制起源.

主要方法:

对Saccharomyces cerevisiae端粒的DNA序列分析.
在相关的酵母物种之间进行比较序列分析.

主要成果:

确定了三个中度重复的元素:131 (1-1.5 kb),Y (5.2 kb) 和X (0.3-3.75 kb).
发现了高密度的复制起源 (约. 在 X 或 Y 序列内).
观察到大约131-Y单元和异质终端T序列 (0.33-0.6 kb) 的合排列.
已确定的端粒序列顺序:T-(Y-131) n-X-,其中n=1-4.
在S. cerevisiae菌株中发现保护性,但在密切相关的物种中存在分歧.

结论:

细菌菌的端粒具有复杂的,保存的结构,具有众多的复制起源.
端粒序列组织和保存模式为酵母基因组进化提供了洞察力.

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