在现场杂交以研究已识别的神经元的起源和命运
Science (New York, N.Y.)
|November 18, 1983
概括
在Aplysia中产卵激素 (ELH) 基因在广泛的神经网络中表达,而不仅仅是袋细胞. 这些神经元起源于人体壁面.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 在Aplysia中,产卵行为受到神经的调节,包括产卵激素 (ELH).
- ELH是由一个称为袋细胞的特定神经元集群释放的.
- 已经确定了编码ELH和相关的基因家族.
研究的目的:
- 确定表达ELH基因家族的神经元的起源和分布.
- 研究这些神经元在Aplysia中枢神经系统中的发育迁移.
主要方法:
- 神经基因家族的分离和核酸测序.
- 在现场杂交以检测基因表达模式.
- 免疫光染色以可视化神经元分布.
主要成果:
- 该ELH基因表达在一个广泛的神经系统在四个中枢神经系统的五个,超出袋细胞.
- ELH基因的表达发生在动物生命周期的早期.
- 表达ELH的神经元起源于体壁的外皮,并迁移到中枢神经系统.
结论:
- 该ELH基因家族在一个比以前理解的更广泛的神经网络中表达.
- 表达ELH的神经元表现出从外皮到中枢神经系统的迁移性发育途径.
- 这项研究揭示了对产生类神经元的神经发育起源的新见解.
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