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相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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相关实验视频

Updated: May 12, 2026

Purification of Transcripts and Metabolites from Drosophila Heads
12:49

Purification of Transcripts and Metabolites from Drosophila Heads

Published on: March 15, 2013

一个Drosophila代谢基因转录被替代处理.

S Henikoff, J S Sloan, J D Kelly

    Cell
    |September 1, 1983
    PubMed
    概括

    研究人员研究了Drosophila纯素通路酶GAR转化酶. 一种新的外基因映射方法揭示了一个单一的基因可以通过替代性转录处理产生两个不同的多,潜在地指导代谢流.

    科学领域:

    • 分子生物学分子生物学
    • 生物化学 生物化学
    • 遗传学 遗传学 是一个

    背景情况:

    • 精氨酸通路对于细胞代谢至关重要.
    • GAR转化酶是氨酸生物合成中的一个关键酶.
    • 了解基因转录和蛋白质表达对于像Drosophila这样的模型生物来说至关重要.

    研究的目的:

    • 为了确定编码GAR转基因酶的DrosophilaDNA段的组织和转录.
    • 调查GAR转化酶和相关蛋白质的产生背后的分子机制.
    • 探索替代性转录处理在代谢途径调节中的潜力.

    主要方法:

    • 对于罕见的转录而言,没有cDNA隔离的新型外显子映射技术.
    • 对DrosophilaDNA片段及其对应的转录进行分析.
    • 生物信息分析用于预测多结构和域.

    主要成果:

    • 确定了一个编码GAR转化酶的DNA段,这是一种 purin通路酶.
    • 发现了一种罕见的转录,在COOH末端编码一个长聚与GAR转化酶.
    • 发现一个单一的基因,通过替代拼接和一个内部的多化信号,从不同的转录产生两个不同的多.

    更多相关视频

    Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics
    07:21

    Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics

    Published on: June 6, 2018

    Measuring O2 Consumption in Drosophila melanogaster Using Coulometric Microrespirometry
    07:12

    Measuring O2 Consumption in Drosophila melanogaster Using Coulometric Microrespirometry

    Published on: July 7, 2023

    相关实验视频

    Last Updated: May 12, 2026

    Purification of Transcripts and Metabolites from Drosophila Heads
    12:49

    Purification of Transcripts and Metabolites from Drosophila Heads

    Published on: March 15, 2013

    Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics
    07:21

    Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics

    Published on: June 6, 2018

    Measuring O2 Consumption in Drosophila melanogaster Using Coulometric Microrespirometry
    07:12

    Measuring O2 Consumption in Drosophila melanogaster Using Coulometric Microrespirometry

    Published on: July 7, 2023

    结论:

    • 单个基因转录的替代处理允许产生两个不同的多.
    • 这种机制可以使代谢中间体进入不同的途径.
    • 这些发现提供了对Drosophila复杂基因表达和代谢流量的复杂调节的见解.