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Updated: Jul 30, 2026

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
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Published on: February 9, 2015

克隆表达和编码人类生长激素释放因子cDNA的序列

K E Mayo, W Vale, J Rivier

    Nature
    |November 3, 1983
    PubMed
    概括

    研究人员确定了人类生长激素释放因子 (hGRF) 的基因,这是一种刺激生长激素释放的类激素. 这一发现澄清了hGRF的结构和起源,这对于理解垂体腺功能至关重要.

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    Recombinant DNA01:09

    Recombinant DNA

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    RACE - Rapid Amplification of cDNA Ends02:35

    RACE - Rapid Amplification of cDNA Ends

    Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
    Since the...