相关实验视频

Updated: Apr 7, 2026

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
13:52

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae

Published on: July 9, 2013

10.8K

hsp70:在环境压力期间的核度和恢复期间的细胞质储存

J M Velazquez, S Lindquist

    Cell
    |March 1, 1984
    PubMed
    概括

    热中的主要热冲击蛋白70 (hsp70) 在热冲击或无氧期间集中在细胞核中,保护细胞免受压力. 这种核转位与hsp70的保护功能有关.

    更多相关视频

    Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules09:33

    Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

    Published on: May 12, 2017

    15.3K
    Measurements of Physiological Stress Responses in C. Elegans10:36

    Measurements of Physiological Stress Responses in C. Elegans

    Published on: May 21, 2020

    15.3K

    相关概念视频

    Nuclear Protein Sorting01:34

    Nuclear Protein Sorting

    Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
    Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
    6.7K
    Regulation of Nuclear Protein Sorting01:45

    Regulation of Nuclear Protein Sorting

    Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
    3.5K
    Export of Misfolded Proteins out of the ER01:32

    Export of Misfolded Proteins out of the ER

    After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
    5.7K
    Regulation of the Unfolded Protein Response01:31

    Regulation of the Unfolded Protein Response

    Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
    3.2K
    Bacterial Protein Maturation01:26

    Bacterial Protein Maturation

    Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
    711
    Other Stress Responses in Bacteria01:30

    Other Stress Responses in Bacteria

    Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
    533