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分子克隆和基因的选择调节在Aspergillus发展中的基因

C R Zimmermann, W C Orr, R F Leclerc

|October 1, 1980

概括

研究人员在Aspergillus nidulans中发现了350多个基因克隆,与多基化 (poly(A) +) RNA相关. 这些RNA在化过程中更为丰富,这表明在真菌发育过程中存在特定的基因调节.

科学领域:

分子生物学分子生物学
菌类学菌类学是指菌类学.
遗传学遗传学是一个

背景情况:

聚基化 (poly(A) +) RNA 在基因表达调节中起着至关重要的作用.
菌的发展,如阿斯伯吉卢斯尼杜兰斯的化,涉及到基因表达的复杂变化.
了解发育调节的遗传基础是真菌生物学的关键.

研究的目的:

为了识别和描述编码多类A) +RNA的基因,这些基因在Aspergillus nidulans的结合过程中特别升级.
研究阿斯伯吉卢斯基因组内的发育控制基因的分布和调节.

主要方法:

从Aspergillus nidulans核DNA构建一个重组DNA库.
使用减法杂交和现场杂交,选择与发育调节的多类A) +RNA同类的克隆.
通过分析不同细胞类型中编码RNA的流行程度和性质,对选择的克隆进行表征.

主要成果:

他们分离了超过350个与发育调节的多A) +RNA同源的克隆.
进一步鉴定了5个克隆细胞,其中4个克隆细胞显示RNA患病率至少增加了10倍.
一个克隆杂交到一个单一的,发育调节的RNA,而另外三种杂交到不同分子重量的多个RNA.

结论:

该研究确定了与Aspergillus nidulans的真菌发育相关的特定RNA种群.
这些发现表明,编码发育调节的多类A) +RNA的基因可能不会随机分布在阿斯伯吉路斯基因组中.
这项研究提供了关于真菌形态发生的基础分子机制的见解.

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