概括
猫类白血病病毒伪型的Moloney肉瘤病毒在转变的细胞中产生p60多蛋白,而不是小鼠p30. 这种p60蛋白是由病毒基因组指定的,在病毒释放之前被低效地分裂.
科学领域:
- 复原病毒研究研究.
- 分子病毒学分子病毒学.
- 瘤发生的发生因子.
背景情况:
- 鼠类p30以前被确定为猫类白血病病毒伪型的莫洛尼肉瘤病毒 (m1MSV) 的组成部分.
- S + L-细胞的抗原反应性归因于小鼠p30.
研究的目的:
- 为了研究莫洛尼肉瘤病毒 (MSV) 转化细胞的多组成.
- 为了确定p60多蛋白是否被MSV基因组指定的.
- 为了阐明与p30生产有关的p60的加工和裂变.
主要方法:
- 使用免疫沉检测五种哺乳动物物种MSV转化细胞中的多.
- 脉冲追踪研究分析细胞内多合成和加工.
- 在不同MSV菌株 (m1MSV,m3MSV,HT-1MSV) 中对多表达的比较.
主要成果:
- 在多个物种的m1MSV转化细胞中检测到了p60多蛋白,而不是小鼠p30.
- p60是由m1MSV基因组指定,是脉冲标记过程中合成的最大的多.
- 在追逐期间,细胞内p60表现出不高效的分裂成像p30这样的较小的多,而p30在病毒释放之前在细胞外出现.
- 在m3MSV和HT-1MSV转化细胞中观察到不同的多形状 (p60,p70,或没有),表明遗传差异.
结论:
- p60多蛋白是m1MSV基因组指定的主要翻译产物.
- 切割p60到p30是一个低效的细胞内过程,发生在晚期,可能是病毒退出之前.
- 在MSV菌株之间的多表达的差异表明它们的遗传构成和瘤潜在的变异.
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