相关实验视频
Updated: Jul 28, 2026

09:04
In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
Published on: May 11, 2020
概括
这项研究开发了一种新型的狂犬病疫苗,使用活的疫苗病毒 (VV) 重组. 新疫苗成功诱导狂犬病病毒中和抗体,并保护小鼠免受狂犬病挑战.
科学领域:
- 病毒学 病毒学
- 疫苗学 疫苗学 疫苗学
- 分子生物学分子生物学
背景情况:
- 尽管存在疫苗,狂犬病仍然是一个重大的全球卫生挑战.
- 目前通过组织培养获得的狂犬病疫苗是有效的,但成本高昂.
- 活体疫苗病毒 (VV) 复合物显示出开发新型疫苗的前景.
研究的目的:
- 开发一种新的,可能更容易获得的狂犬病疫苗.
- 利用疫苗病毒 (VV) 重组技术进行狂犬病免疫.
主要方法:
- 修改的狂犬病糖蛋白cDNA被设计并插入到一个VV胺基因酶基因中.
- 重组VV表达狂犬病糖蛋白是通过同源重组生成的.
- 重组VV狂犬病疫苗的疗效在子和小鼠中进行了评估.
主要成果:
- 给子注射复合VV诱导狂犬病病毒中和抗体的高标位.
- 在小鼠中,用重组VV进行化提供了对街头狂犬病病毒挑战的保护.
结论:
- 活疫苗病毒 (VV) 重组剂可以有效地被设计成表达狂犬病抗原.
- 这种新型重组VV狂犬病疫苗候选人证明了免疫性和保护性疗效.
相关概念视频
Rab Proteins
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rabies
Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...

