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移植抗原的表达和功能与改变或删除的细胞质域

M C Zuniga, B Malissen, M McMillan

|September 1, 1983

概括

改变H-2Ld移植抗原的细胞质域不会影响其细胞表面表达或功能. 这些发现表明,C端残留物对于H-2Ld分子运输,膜融合或T细胞识别并不重要.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

一类移植抗原,如H-2Ld,在免疫反应中起着至关重要的作用.
一类分子的细胞质域参与细胞信号传递和蛋白质贩运.
了解H-2Ld的功能域对于剖析T细胞介导的细胞毒性至关重要.

研究的目的:

研究H-2Ld分子在细胞表面表达和功能中的细胞质域的作用.
确定是否需要特定的C端残留物用于H-2Ld传输,膜融合和T细胞识别.

主要方法:

构建两个H-2Ld突变体,细胞质域发生变化 (删除或替换C端残留物).
突变H-2Ld基因的DNA介导基因转移到小鼠L细胞中.
在T细胞中介细胞毒性试验中分析突变H-2Ld表达,细胞表面定位和功能活性.

主要成果:

突变的H-2Ld基因在L细胞的细胞表面在正常水平上被成功表达.
改变的H-2Ld分子表现出与工程修改一致的电荷特性和大小.
这些突变的H-2Ld分子有效地作为异性细胞毒性T细胞的向抗原,并作为病毒特异性细胞毒性T细胞的限制元素.

结论:

H-2Ld分子的细胞质域的C端残留物对于其运输到血或集成至关重要.
这些C端残留物对于H-2Ld分子作为向抗原和T细胞中介细胞毒性的限制因素的功能也不可或缺.
该研究确定了特定的C端残留物对H-2Ld表面表达和免疫识别的非关键性.

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