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间隔序列调节了注入到Xenopus胚胎中的核糖体基因质粒的转录

S J Busby, R H Reeder

|October 1, 1983

概括

上游间隔序列显著影响Xenopus laevis的核糖体DNA (rDNA) 转录. 删除这些序列会减少基因表达,这表明它们调节促进体激活频率.

科学领域:

分子生物学分子生物学
发展生物学发展生物学
遗传学是一个遗传学.

背景情况:

核糖体DNA (rDNA) 转录对于细胞生长和发育至关重要.
rDNA基因的促进子区域控制着转录的启动.
之前的研究已经确定了Xenopus laevis rDNA中的核心促进体.

研究的目的:

研究上游间隔序列在调节Xenopus laevis rDNA转录中的作用.
为了确定远离促进子的序列是否会影响rDNA基因表达水平.

主要方法:

在受精卵中注射含有Xenopus laevis rDNA重复单位的等离子体.
从注射的等离子体中分析转录启动时间和水平.
上游间隔序列的删除分析,以评估它们对转录的影响.

主要成果:

从注射的rDNA等离子体中转录在晚期芽细胞阶段与内源rDNA基因同时启动.
删除上游间隔序列 (超过1150bp) 将rDNA转录减少5至10倍.
距离促进子远远的间隔序列显著影响rDNA基因表达水平.

结论:

上游间隔序列在调节rDNA促进子激活的频率方面发挥着至关重要的作用.
这些遥远的调节元素对于达到最佳的rDNA基因转录水平至关重要.
这些发现揭示了Xenopus laevis.中rDNA基因表达的复杂调节机制.

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