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相关概念视频

Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
Transcription01:17

Transcription

Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

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相关实验视频

Updated: Jul 17, 2026

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

在正常胚胎发育和瘤转化过程中调节的转录具有共同的重复元素.

D Murphy, P M Brickell, D S Latchman

    Cell
    |December 1, 1983
    PubMed
    概括

    集合1重复元素在小鼠胚胎中识别出发育调节的转录. 在多能细胞系中分化后,它们的丰度会减少,除了F9细胞.

    科学领域:

    • 分子生物学分子生物学
    • 发育生物学 发展生物学
    • 基因组学就是基因组学.

    背景情况:

    • 之前分离的cDNA克隆与转化小鼠纤维细胞中的升高mRNA相同.
    • 一组克隆含有分散的重复元素,在小鼠基因组中发现了数千次.

    研究的目的:

    • 研究一组1重复元素在小鼠发育过程中的基因调节中的作用.
    • 在胚胎和细胞系模型中分析Set 1相关转录的表达模式.

    主要方法:

    • 隔离对特定mRNA同异的cDNA克隆.
    • 在小鼠基因组中对重复元素分布的分析.
    • 使用杂交技术对RNA转录的量化.
    • 胚胎组织和细胞系中转录模式的比较 (EC,EK,F9).

    主要成果:

    • 集合1重复在小鼠胚胎中识别了许多量化调节的转录,在高峰表达时构成1-3%的多基化RNA.
    • 在多能EC和EK细胞系中观察到与中期胚胎类似的转录模式.
    • 在EC和EK细胞的体外分化后,RNA的丰度下降.
    • 具有有限发育能力的F9 EC细胞系表现出更简单的Set 1转录模式.

    更多相关视频

    LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
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    LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

    Published on: February 1, 2020

    Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
    09:58

    Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

    Published on: June 27, 2020

    相关实验视频

    Last Updated: Jul 17, 2026

    Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
    09:16

    Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

    Published on: September 1, 2019

    LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
    12:18

    LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

    Published on: February 1, 2020

    Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
    09:58

    Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

    Published on: June 27, 2020

    结论:

    • 集合1重复的元素与小鼠的发育调节的转录有关.
    • 这些转录的表达与多能性和分化过程有关.
    • 集合1的转录模式的变化可能反映了细胞系的发育潜力的差异.