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相关概念视频

The Extracellular Matrix01:42

The Extracellular Matrix

Overview
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Glycocalyx and its Functions01:14

Glycocalyx and its Functions

The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
Components of...

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相关实验视频

Updated: May 11, 2026

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

对细胞间LETS糖蛋白质矩阵的研究.

L B Chen, A Murray, R A Segal

    Cell
    |June 1, 1978
    PubMed
    概括
    此摘要是机器生成的。

    小胚胎纤维细胞分泌出细胞外基质,主要由大型外部转化敏感蛋白 (LETS) 组成. 这些LETS蛋白质矩阵由二硫化物键连接在一起,影响细胞形状和肌细胞融合.

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    Published on: April 21, 2017

    Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
    11:21

    Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

    Published on: January 20, 2022

    相关实验视频

    Last Updated: May 11, 2026

    A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
    09:40

    A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

    Published on: January 4, 2017

    Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
    14:02

    Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

    Published on: April 21, 2017

    Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
    11:21

    Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

    Published on: January 20, 2022

    科学领域:

    • 细胞生物学 细胞生物学
    • 生物化学 生物化学
    • 细胞外矩阵研究 细胞外矩阵研究

    背景情况:

    • 细胞与细胞的接触对于小胚胎纤维细胞的细胞外基质形成至关重要.
    • 这些矩阵是纤维状的,单个纤维直径为5-10纳米,它们聚集成捆.

    研究的目的:

    • 研究小胚胎纤维细胞分泌的细胞间基质的组成和特性.
    • 确定大型外部转换敏感 (LETS) 蛋白在矩阵形成和功能中的作用.

    主要方法:

    • 扫描和传输电子显微镜可视化矩阵结构.
    • 免疫光和免疫蛋白技术用于识别蛋白质成分.
    • 用洗剂处理以分离无细胞基质.
    • 使用β-mercaptoethanol进行溶解研究.

    主要成果:

    • LETS蛋白质是主要成分,占无细胞基质的90%以上.
    • 矩阵可能是LETS蛋白质的聚合物,通过二硫化键连接在一起,这是溶解要求所表明的.
    • 原不参与这些矩阵.
    • LETS蛋白质矩阵促进细胞形状变化和肌细胞融合,但不会影响细胞生长,葡萄糖吸收或蛋白酶产生.

    结论:

    • 小胚胎纤维细胞外细胞矩阵主要由LETS蛋白组成,形成二硫化物结合的聚合物.
    • LETS蛋白质矩阵在调节细胞形态和融合方面发挥着重要作用,独立于细胞生长或代谢活动.