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在3T3-adipocytes中,细胞形状和脂质基因表达的纤维素调节3T3-adipocytes

B M Spiegelman, C A Ginty

|December 1, 1983

概括

纤维肌菌素通过抑制脂原酶基因表达来阻止3T3-F442A细胞分化,这表明细胞骨变化对这一过程至关重要.

科学领域:

细胞生物学细胞生物学
生物化学生物化学
分子生物学分子生物学

背景情况:

3T3-F442A细胞分化涉及形态变化和改变的蛋白质合成.
在分化过程中,细胞骨蛋白质合成减少,而脂原酶生物合成增加.

研究的目的:

调查纤维素在3T3-F442A细胞分化过程中调节基因表达中的作用.
确定纤维素对脂质酶诱导和细胞骨变化的影响.

主要方法:

在纤维菌素矩阵上培养3T3-F442A细胞.
测量蛋白质生物合成和mRNA水平.
对细胞形态和对细胞素D和胰岛素的反应进行分析.

主要成果:

纤维素素可以防止形态变化,并减少脂质酶诱导 (糖酸脱酶,脂肪酸合成酶).
纤维肌菌素不会影响与分化相关的actin和tubulin生物合成下降.
抑制脂原蛋白生物合成是由于减少了特定的mRNA水平.
通过维持细胞圆形或使用细胞素D或胰岛素,纤维素素的抑制作用是可逆的.

结论:

纤维蛋白在细胞分化过程中调节脂原蛋白的基因表达.
纤维肌菌素干扰了脂质基因表达所需的基本细胞骨和形态变化.

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