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2型腺病毒的完整转化需要两种E1A蛋白

C Montell, G Courtois, C Eng

|April 1, 1984

概括

这两种腺病毒早期区域1A (E1A) 蛋白都对完全细胞转化至关重要. 289氨基酸蛋白和243氨基酸蛋白合作,在特定细胞类型中实现完整的转化和有效的病毒复制.

科学领域:

分子生物学分子生物学
病毒学病毒学
细胞生物学细胞生物学

背景情况:

2型腺病毒 (Ad2) 使用早期区域1A (E1A) 编码的蛋白质转化动物细胞.
两个高度相关的E1A蛋白质,长度为289和243氨基酸,在Ad2转化细胞中表达.

研究的目的:

研究289和243氨基酸E1A蛋白在腺病毒介导的细胞转化中的不同作用.
为了确定每个E1A蛋白质对 anchorage独立生长和病毒复制的要求.

主要方法:

使用了表达289或243氨基酸E1A蛋白的腺病毒突变.
评估了转变的表型,包括不依赖定位的生长.
在标准和增长停止的人类细胞中评估病毒复制效率.

主要成果:

两个E1A蛋白对于完全转变的表型是必要的;每一个单独都会诱导部分转变.
243 个氨基酸E1A 蛋白质对于无成长至关重要.
仅仅由243氨基酸E1A蛋白调节的转化是对寒冷敏感的.
在增长停止的人类细胞中有效的病毒复制需要两种E1A蛋白质,而在标准细胞培养中,只有289氨基酸蛋白质足以复制.

结论:

289和243氨基酸E1A蛋白质协作,以实现完全的腺病毒诱导的细胞转化.
243氨基酸E1A蛋白质在特定细胞环境中对结独立生长和有效的病毒复制起着至关重要的作用.

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