概括
鼠下腺 (SMG) 氨酸水平的遗传调节是由氨酸调节部位 (Rnr) 控制的. 这种调节发生在信使RNA (mRNA) 度水平上,具有明显的DNA模式,表明高素菌株中的基因重复.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 蛋白调节部位 (Rnr) 影响小鼠下腺 (SMG) 的蛋白水平.
- 之前的研究表明,对SMG蛋白水平的遗传控制.
研究的目的:
- 为了确定SMGrenin遗传调节的分子基础.
- 为了研究Rnr等位基因和雷宁mRNA水平之间的关系.
- 分析不同Rnr菌株的DNA序列组织.
主要方法:
- 在体外翻译以识别SMG雷宁聚.
- 隔离了一个特定的SMG雷宁cDNA重组克隆.
- 北方斑点分析以量化宁mRNA度.
- 南方斑点分析检查DNA序列组织.
主要成果:
- 一个45,000达尔顿的多被确定为SMGrenin.
- 基因调节SMG雷宁水平与雷宁mRNA度直接相关.
- 在高素 (Rnrs) 和低素 (Rnrb) 菌株之间观察到明显的DNA限制模式,这表明Rnrs.中的结构基因重复.
- 基因剂量不能解释Rnrs和Rnrb菌株之间SMG蛋白水平的显著 (高达100倍) 差异.
结论:
- 氨酸调节位 (Rnr) 主要通过调节氨酸mRNA度来控制SMG氨酸水平.
- 证据表明,在高氨酸的小鼠菌株中,Rnr位点存在结构性基因复制.
- 高蛋白菌株中蛋白水平急剧增加的机制需要进一步研究,而不仅仅是简单的基因剂量.
更多相关视频
05:46Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
Published on: June 9, 2020
06:48Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
Published on: April 29, 2022
相关概念视频
Gene Families
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Multi-species Conserved Sequences
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Gene Duplication and Divergence
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.
Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
