Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Ratio of X Chromosome to Autosomes02:45

The Ratio of X Chromosome to Autosomes

11.5K
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.  
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
11.5K
Diversity of Protists IV01:27

Diversity of Protists IV

2.1K
Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...
2.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Role of vitamin d on the inhibition of gastrin production after Cisplatin treatment.

Metal-based drugs·2008
Same author

The genome of the social amoeba Dictyostelium discoideum.

Nature·2005
Same author

Cross-induction of cell types in Dictyostelium: evidence that DIF-1 is made by prespore cells.

Development (Cambridge, England)·2001
Same author

The role of DIF-1 signaling in Dictyostelium development.

Molecular cell·2001
Same author

Cell-fate choice in Dictyostelium: intrinsic biases modulate sensitivity to DIF signaling.

Developmental biology·2000
Same author

Ca(2+) signalling is not required for chemotaxis in Dictyostelium.

The EMBO journal·2000

相关实验视频

Updated: May 5, 2026

RNAi Interference by dsRNA Injection into Drosophila Embryos
08:30

RNAi Interference by dsRNA Injection into Drosophila Embryos

Published on: April 11, 2011

15.7K

缺乏DIF的Dictyostelium突变体,这是一个假定的形态原体.

W Kopachik, A Oohata, B Dhokia

    Cell
    |June 1, 1983
    PubMed
    概括
    此摘要是机器生成的。

    细胞粘液模具的分化依赖于分化诱导因子 (DIF). 缺少DIF可以防止茎细胞的形成,在Dictyostelium discoideum中停止形前的发展.

    更多相关视频

    Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes
    18:38

    Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

    Published on: September 26, 2013

    11.5K
    Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
    06:08

    Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

    Published on: January 25, 2019

    14.9K

    相关实验视频

    Last Updated: May 5, 2026

    RNAi Interference by dsRNA Injection into Drosophila Embryos
    08:30

    RNAi Interference by dsRNA Injection into Drosophila Embryos

    Published on: April 11, 2011

    15.7K
    Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes
    18:38

    Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

    Published on: September 26, 2013

    11.5K
    Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
    06:08

    Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

    Published on: January 25, 2019

    14.9K

    科学领域:

    • 细胞和发育生物学
    • 生物化学 生物化学
    • 分子信号传递是分子信号传递.

    背景情况:

    • 分化诱导因子 (DIF) 是一种内生细胞外信号.
    • DIF是一种可透析的,类似脂质的因子,可以控制Dictyostelium discoideum (D. discoideum) 细胞分化.
    • 在与cAMP化的孤立D. discoideum amebae中,DIF诱导了茎细胞的形成.

    研究的目的:

    • 调查DIF剥夺对D. discoideum发育的影响.
    • 为了描述在DIF积累中受损的突变菌株.
    • 了解DIF在细胞分化和形态发育中的作用.

    主要方法:

    • 隔离和特征 D. discoideum 突变菌株与受损的 DIF 积累.
    • 在体外测试以评估茎细胞的形成.
    • 在活体中对亚格拉菌体的形态发育进行了研究.
    • 通过外源DIF添加来纠正突变表型.

    主要成果:

    • 突变菌株积累了很少的DIF.
    • 在试验室中,突变细胞的茎细胞形成受损.
    • 在聚合后停止的阿加尔上的形态发育,在形成之前.
    • 外源DIF添加纠正了体外和体内缺陷.
    • 前细胞分化发生,但在缺乏DIF的条件下,前细胞分化被抑制.

    结论:

    • 在D. discoideum. 中,DIF对于前细胞分化至关重要.
    • DIF的剥夺导致了形成之前的发育停滞.
    • 缺乏DIF的突变菌株为研究细胞分化途径提供了宝贵的工具.