概括
大斑块 (Ip) 突变的腺病毒2在细胞转化中有缺陷. 在19kd瘤抗原中的突变.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 腺病毒2大斑块 (Ip) 突变在人类的 KB 细胞上形成大透明斑块.
- 这些突变者在诱导大鼠胚胎细胞系3Y1.1的转化方面表现出缺陷.
- 转化早期基因块E1b (地图位置4.5-11.2) 编码了53kd和19kd的T抗原.
研究的目的:
- 为了研究亚地诺病毒2大斑块 (Ip) 突变体在细胞转化中的作用.
- 为了在E1b转换基因块内定位Ip突变.
- 分析19 kd瘤抗原中的特定突变缺陷及其对转化的影响.
主要方法:
- 标记物转移以在E1b基因块内定位Ip突变.
- 对Ip突变 (Ip3和Ip5) 的DNA序列分析.
- 在受影响的突变体中分析病毒E1蛋白质.
主要成果:
- 基因突变局限于E1b块内的19kd瘤抗原编码区域.
- 突变Ip3在19kd多的N端表现出单个基对的变化,将氨酸改变为氨酸.
- 突变Ip5有两种突变:一种是将酸改变为N端附近的氨酸,另一种是通过改变一个停止编码子来扩展19kd多.
结论:
- 直接的遗传证据证实了 19 kd 瘤抗原在 Adenovirus 2 诱导的细胞转化中的重要作用.
- 19kd瘤抗原的N终端区域对于其诱导细胞转变的功能至关重要.
- 19kd抗原的特定变化,包括N端变化和尺寸修改,损害了它的转化活性.
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