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由血小板衍生生长因子调节的基因序列的分子克隆

B H Cochran, A C Reffel, C D Stiles

|July 1, 1983

概括

血小板衍生生长因子 (PDGF) 在BALB/c-3T3细胞中直接调节早期基因KC和JE. 这些基因在PDGF刺激时显示出快速,显著的mRNA诱导,独立于蛋白质合成.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
遗传学是一个遗传学.

背景情况:

血小板衍生生长因子 (PDGF) 是一种强大的线粒原体,参与细胞生长和增殖.
了解生长因子对基因调节的理解对于破译细胞反应至关重要.

研究的目的:

在BALB/c-3T3细胞中直接受PDGF调节的基因序列的识别和特征.
为了研究PDGF诱导的基因表达的动力学和特异性.

主要方法:

从BALB/c-3T3细胞中选一个cDNA库.
混合选择和翻译以识别编码的多.
在PDGF刺激后对mRNA丰度变化的定量分析.
测试与无关的线粒激素和蛋白质合成抑制剂的作用.

主要成果:

从8000个克隆中确定了不到14个独立的PDGF诱导序列.
对编码10kDa和19kDa多的KC和JE序列的详细表征.
在1小时内观察到10到20倍的mRNA诱导,在4小时内达到JE的60倍.
证明了PDGF的特异性,EGF,胰岛素或血的影响最小;蛋白质合成抑制剂没有阻止诱导.

结论:

KC和JE被认为是PDGF直接调节的"早期基因".
PDGF诱导的基因表达发生得很快,并且独立于新的蛋白质合成.
这些发现为PDGF信号触发的直接分子事件提供了洞察力.

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