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相关概念视频

Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

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Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
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Mitochondria01:37

Mitochondria

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Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
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Mitochondrial Membranes01:45

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A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
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相关实验视频

Updated: Apr 2, 2026

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
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Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

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一些酵母线粒体RNA是圆形的.

A C Arnberg, G J Van Ommen, L A Grivell

    Cell
    |February 1, 1980
    PubMed
    概括

    酵母线粒体RNA包括圆形分子,其中11S和18S分数显示不同的大小. 这些圆形RNAs是共封闭的,可以作为信使RNA或RNA处理.

    科学领域:

    • 线粒体遗传学线粒体遗传学
    • RNA生物学的RNA生物学
    • 分子生物学分子生物学

    背景情况:

    • 酵母线粒体含有各种RNA物种.
    • 这些RNA的结构形式尚未完全理解.
    • 在其他系统中已经观察到圆形RNA分子.

    研究的目的:

    • 为了研究酵母线粒体中圆形RNA分子的存在和特征.
    • 确定这些循环RNA的结构性质和潜在功能.

    主要方法:

    • 使用 agarose 凝电泳,分离酵母线粒体RNA 分数 (11S 和 18S).
    • 电子显微镜用于可视化和测量圆形RNA分子.
    • 使用特定的线粒体DNA片段进行RNA-DNA杂交实验.
    • 酶处理 (DNAase,pronase) 和化学处理 (glyoxal) 来评估结构完整性.

    主要成果:

    • 大约50%的11S和18S酵母线粒体RNA分离物以圆形分子的形式存在.
    • 11S圆的轮长度为0.36±0.02微米,而18S圆的平均长度为0.78±0.11微米.
    • 11S圆形RNA与线粒体DNA的oxi3区域形成稳定的R环和RNA-DNA混合体,这表明它在编码细胞氧化酶子单元1中的作用.

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  • 11S和18S圆形RNA都耐酶和化学处理,这表明它们是共封闭结构.

    • 结论:

    • 酵母线粒体RNA含有很大比例的共封闭圆形分子.
    • 这些循环RNAs,特别是11S分数,与oxi3基因区域有关.
    • 这些循环RNA的确切功能尚不清楚,但可能涉及作为信使RNA,存储分子或RNA处理中的中间体的作用.