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在海RNA中克隆的重复DNA序列的特定表示

R H Scheller, F D Costantini, M R Kozlowski

|September 1, 1978

概括

重复序列的基因表达在海胚胎和成年细胞之间有所不同. 这突出了细胞特异性模式在核RNA中重复的DNA序列的表示.

科学领域:

分子生物学分子生物学
发展生物学发展生物学
基因组学就是基因组学.

背景情况:

在真核生物基因组中,重复性的DNA序列是丰富的.
重复序列及其转录的功能意义尚未完全理解.
不同基因表达对细胞分化和发育至关重要.

研究的目的:

为了研究不同类型的海细胞核RNA中克隆的重复DNA序列的定量表示.
为了确定重复序列转录模式是否是细胞特异的.
探索重复序列与核RNA复杂性之间的关系.

主要方法:

标记的克隆重复序与来自海胃胚胎和成年肠道的RNA混合.
使用RNA过量杂交动力学和DNA过量定位测量转录度.
对线程表示和转录大小的分析.

主要成果:

观察到不同重复序列家族的转录水平有显著的定量变化.
高度表示的重复序列的集合在胃肠和肠核RNA之间有所不同.
所有克隆重复的两个链都被转录,每个链的水平一般都相似.

结论:

核RNA中重复序列表示的定量模式是特定于细胞类型的.
这种差异表示表明重复序列在细胞特异性核RNA代谢中的作用.
重复序列的转录在核RNA中发现,而不是多体信使RNA,并且是大型分子.

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