相关实验视频

Updated: Aug 14, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

用单克隆抗体定义的几内亚猪精子的表面域

D G Myles, P Primakoff, A R Bellvé

|February 1, 1981

概括

研究人员开发了四种单克隆抗体,识别了几内亚猪精子上独特的表面分子. 这些抗体揭示了精子表面的不同领域,有助于理解精子细胞的分化和结构.

科学领域:

生殖生物学生殖生物学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

精子细胞的表面含有对于受精至关重要的分子.
了解精子表面异质性是生殖研究的关键.

研究的目的:

产生和表征针对几内亚猪精子表面分子的单克隆抗体.
识别和绘制几内亚猪精子表面上不同的域名.

主要方法:

对几内亚猪精子产生四种单克隆抗体.
抗体结合测试以确定精子表面的局部.
吸收实验以确定抗原类型.
免疫沉和SDS-PAGE用于确定抗原的分子量.

主要成果:

四个单克隆抗体在精子表面上识别出独特的局部区域:前头,后头,整个头和后尾.
已识别的分子被确定为差异化抗原.
三种抗体免疫降落蛋白质抗原的分子重量为52,000,42,000和60,000Da.

结论:

几内亚猪的精子表面分为至少四个不同的领域.
这些域是由特定的分化抗原定义的,为精子细胞的发育和功能提供了洞察力.

更多相关视频

Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses

Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses

Published on: October 7, 2020

Recording Electrical Currents across the Plasma Membrane of Mammalian Sperm Cells

Recording Electrical Currents across the Plasma Membrane of Mammalian Sperm Cells

Published on: February 14, 2021

相关概念视频

Cross-reactivity

Cross-reactivity

Fertilization

Fertilization

During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...