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相关概念视频

Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Termination of Translation01:44

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
Transcription01:17

Transcription

Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Termination of Translation01:44

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...

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相关实验视频

Updated: May 7, 2026

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
11:21

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

tyrT位点:一个复杂的转录的终结和处理.

J Rossi, J Egan, L Hudson

    Cell
    |November 1, 1981
    PubMed
    概括
    此摘要是机器生成的。

    大肠杆菌 tyrT 位有一个带有重复序列和终结点的间隔区域. 一种对温度敏感的rho蛋白会影响转录的读透,揭示tRNA和蛋白质的双重功能转录.

    更多相关视频

    Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
    12:12

    Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

    Published on: March 12, 2017

    DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
    09:26

    DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

    Published on: December 29, 2021

    相关实验视频

    Last Updated: May 7, 2026

    Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
    11:21

    Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

    Published on: September 10, 2017

    Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
    12:12

    Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

    Published on: March 12, 2017

    DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
    09:26

    DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

    Published on: December 29, 2021

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 微生物学 微生物学

    背景情况:

    • 大肠杆菌 tyrT 位对tRNATyr1的两个副本进行编码,它们被一个间距区域分开.
    • 这个间隔器包含了与在 tyrT locus.下游发现的重复相同的序列.

    研究的目的:

    • 研究大肠杆菌TyrT位点的结构和功能.
    • 分析转录终结点和主要转录的性质.
    • 探索编码tRNA和蛋白质的双重功能转录的潜力.

    主要方法:

    • 分析 tyrT 位序列和结构.
    • 使用温度敏感rho蛋白对转录终结的研究.
    • 主要转录的识别和表征.

    主要成果:

    • tyrT位点包含了一组分级的转录终结位点,间隔180个基数.
    • 主要终结站点位于第二个重复单元内.
    • 在这个位置,温度敏感的rho蛋白的读透率增加,导致下游站点的终止.
    • 主要转录编码了一个低分子量,基本蛋白质,由于基因替代,假基因在类似的序列中编码.

    结论:

    • tyrT位点表现出复杂的结构,具有双重功能转录的潜力.
    • 转录调节受到rho蛋白和终结位的影响.
    • 间隔器和重复中的同源序列可能代表蛋白质编码的伪基因.