编码Saccharomyces cerevisiae的交配类型位置的DNA序列
Cell
|November 1, 1981
概括
研究人员测序了酵母交配类型的MATA和HMLα位点,揭示了独特的DNA段和mRNA编码. 蛋白质预测表明a1蛋白质有一个令人惊的读透机制.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 基因组学就是基因组学.
背景情况:
- 酵母交配类型的位控制着性繁殖.
- 了解MATa和HMLα序列对于破译交配类型决定至关重要.
研究的目的:
- 为了确定酵母MATa和HMLα位点的完整DNA序列.
- 分析编码的mRNA和预测蛋白质序列.
主要方法:
- 对MATa和HMLα的DNA测序.
- mRNA-DNA对齐. mRNA-DNA对齐. 这是一个很好的方法.
- 从DNA中预测蛋白质序列.
主要成果:
- 确定了MATa (642 nt独特部分) 和HMLα (747 nt独特部分) 的完整序列.
- 将a1,a2,alpha1和alpha2mRNA与DNA序列对齐.
- 预测的a1蛋白 (148个氨基酸) 由UGA读透而产生的;α1和α2mRNA的转录方式不同.
结论:
- 这项研究阐明了酵母交配类型局部的遗传组织.
- 确定了独特的DNA段和mRNA编码策略.
- 表明交配型切换边界与特定的DNA序列相邻.
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