在转录终结和RNA处理层面调节腺病毒-2基因表达
Nature
|March 12, 1981
概括
腺病毒-2 转录显示了动态调节. 在感染早期,终结和RNA处理不同,通过改变L1mRNA生产来影响基因表达.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 基因表达规范 基因表达规范
背景情况:
- 腺病毒-2 主要晚期转录单元对于病毒复制至关重要.
- 了解它在不同感染阶段的活动是破译病毒基因表达策略的关键.
研究的目的:
- 为了研究腺病毒-2主要晚期转录单元的时间调节.
- 阐明转录终止和RNA处理事件在感染的早期和晚期期间如何变化.
主要方法:
- 分析不同感染时间点的腺病毒-2转录模式.
- 检查RNA处理事件,包括选择和拼接多基化位点.
主要成果:
- 主要的晚期转录单元在感染初期是活跃的,类似于其他早期单元.
- 在早期感染中,转录终止发生过早 (地图位置60-70),但在感染晚期的地图位置99发生.
- 在早期感染期间,RNA处理产生单个L1mRNA,与晚期感染不同.
结论:
- 在腺病毒-2 感染期间,转录终结,多元A位点选择和拼接是动态调节的.
- 这些RNA处理和终结的变化有助于控制基于细胞条件的病毒基因表达.
相关概念视频
Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription Attenuation in Prokaryotes
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
What is Gene Expression?
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...


