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Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
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Published on: December 27, 2014

在培养细胞中定位血清衍生的α2宏球蛋白,并在莫洛尼肉瘤病毒转化后减少.

I Pastan, M Willingham, W Anderson

    Cell
    |November 1, 1977
    PubMed
    概括
    此摘要是机器生成的。

    培养的纤维细胞从血清中吸收α2宏球蛋白 (α2M),将其存储在与溶酶体不同的囊泡中. 这种蛋白酶抑制剂可以通过抑制细胞蛋白酶来影响细胞行为.

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    科学领域:

    • 细胞生物学 细胞生物学
    • 生物化学 生物化学
    • 蛋白酶抑制可以抑制蛋白质酶.

    背景情况:

    • 培养的纤维细胞,包括NRK细胞,含有α2宏球蛋白 (α2M).
    • 阿尔法2M是由细胞培养基中存在的小牛血清获得的.
    • 随后,一部分内部化的α2M被释放回介质中.

    研究的目的:

    • 在培养的纤维细胞中研究α2宏球蛋白 (α2M) 的细胞内定位和起源.
    • 为了确定细胞合成的α2M是否释放到培养基中.
    • 探索alpha2M在调解血清对细胞行为影响中的潜在作用.

    主要方法:

    • 使用14C-氨基酸来追踪蛋白质合成的放射标记实验.
    • 光显微镜与罗达胺标记抗体可视化固定细胞中的α2M.
    • 活细胞的光显微镜观察罗达胺标记的α2M的吸收.

    主要成果:

    • 细胞内和细胞外α2M没有显示可检测的放射性,表明它不是由细胞合成的.
    • 阿尔法2M被定位在不同于初级溶解体的囊泡性器官中.
    • 观察到罗达胺标记的α2M被吸收到活细胞中类似的囊泡结构中.
    • 莫洛尼肉瘤病毒转化细胞表现出最低水平的α2M.

    结论:

    • 在培养纤维细胞中发现的α 2 宏球蛋白来源于培养基的血清.
    • 阿尔法2M被隔离在特定的细胞内囊泡中,而不是溶解体.
    • 血清衍生的α2M可能通过蛋白酶抑制调节细胞功能,可能影响细胞行为.