人类β-环球蛋白位置控制区域参与启动DNA复制的过程
M I Aladjem1, M Groudine, L L Brody
1Gene Expression Laboratory, Salk Institute, San Diego, CA 92037, USA.
概括
在人类β-环球蛋白位点中启动DNA复制取决于位点控制区域 (LCR). 复制从LCR的下游50kb开始,需要跨物种保存的远距离序列之间的相互作用.
科学领域:
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 人类β-环球蛋白位置控制区域 (LCR) 对于调节基因表达,染色体组织和DNA复制时间至关重要.
- 了解DNA复制启动的机制对于细胞生物学和细胞发育至关重要.
研究的目的:
- 调查LCR及其相关序列在人类β-环球蛋白位点内启动DNA复制中的作用.
- 为了确定复制启动机制是否在不同物种中保持.
主要方法:
- 对人类,小鼠和细胞中含有人类β-环球蛋白位点的DNA复制启动部位的分析.
- 使用缺少LCR包围序列的β-环球因子的删除突变.
- 研究突变基位中的复制方向性.
主要成果:
- 独立于转录的DNA复制,在LCR的下游50千基位点在完整的位置启动DNA复制.
- 在缺少LCR相关序列的删除突变体中,复制启动失败了.
- 突变基因表现出3'到5'复制,这表明启动中出现了干扰.
结论:
- 包括LCR在内的遥远DNA序列之间的相互作用对于在β-环球蛋白位点启动DNA复制是必不可少的.
- 参与调解这些长距离相互作用以启动复制的因素在进化上是保留的.
相关概念视频
Replication in Prokaryotes
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell’s circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from two replication forks, resulting in two DNA molecules.Many Proteins Work Together to Replicate the ChromosomeReplication is coordinated and carried out by a host of specialized proteins.
Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.Many Proteins Orchestrate Replication at the OriginEukaryotic replication follows many of the same principles...
Replication in Prokaryotes
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell’s circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from two replication forks, resulting in two DNA molecules.Many Proteins Work Together to Replicate the ChromosomeReplication is coordinated and carried out by a host of specialized proteins.
S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Replication in Prokaryotes
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...


