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Updated: Jun 19, 2026

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
11:48

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Published on: July 24, 2013

针对性地破坏神经元中氧化合成酶基因

P L Huang1, T M Dawson, D S Bredt

  • 1Cardiovascular Research Center, Massachusetts General Hospital, Boston 02129.

Cell
|December 31, 1993
PubMed
概括

缺少神经元氧化合成酶 (NOS) 基因的小鼠患有胃膨胀和pyloric关节缩. 这一发现提供了对婴儿pyloric狭窄症的见解,这是一种与NOS神经元缺乏相关的人类疾病.

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Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System11:49

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Published on: June 23, 2023

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What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...